Abstract: A protocol for rapid in vitro propagation of Saccharum officinarum using meristem and axillary buds as explants was developed. Murashige and Skoog (MS) medium supplemented with 20 g l-1 sucrose, 2 g l-1 gelrite and various combination of kinetin (Kn) and gibberrellic acid (GA3) was used as a culture initiation medium. Thirty three percent of the apical buds and 20% of the axillary buds grew into shoots within 15 days on media containing 1 mg l-1 Kn and 0.1 mg l-1 GA3. Transferring the shoots after they were 5cm long on the liquid medium for 15 days led to the maximum multiplication of the shoots. Plantlets with profuse roots were obtained 10 days after the shoots were removed off and planted on the liquid medium containing 60 g l-1 of sucrose. More then 95% of the plantlets survived after one month when they were transplanted in the vermiculite.