Abstract: Genetic control of pigmentation in different parts of cowpea (Vigna unguiculata (L.) Walp.) was studied in six F2 segregating populations and their corresponding F3 families derived from eight parents. Monogenic control for colour expression was found for leaf node pigmentation, flower (petal) colour, immature pod colour, seed coat colour, seed eye colour and seed eye colour pattern. Presence of pigment was dominant over absence of pigment and the black seed eye was dominant over brown eye. For seed eye pattern however, partial dominance of the very small eye type over the Holstein eye type was observed. Different flower colour genes induce similar white flower colours in the parents and non-allelic interactions between these white flower colour loci produced novel flower colour variants in the F2 population. In the parental lines, flower and seed pigmentation are due to the same single locus effects, although flower colour may be influenced by epistatic gene interactions that have no effects on seed coat pigmentation. Pleiotropic effects of the recessive locus that causes loss of pigmentation of the leaf node include loss of pigment in petals and at least, in a genetic background of lines with a white seed coat, induces intense pigmentation around the hilum. By contrast, there are no apparent pleiotropic effects of the pod colour locus and the seed eye colour locus on pigmentation in other tissues.