Abstract: The experiment was undertaken to determine the effect of spacing on onion cultivation of different varieties. Different spacings were taken 20x10 cm, 15x10 cm, 10x10 cm, 15x7.5 cm, 10x7.5 cm and 7.5x7.5 cm. Three varieties viz BARI Piaz-1, Taherpuri and Faridpur Bhati were used for study. Significantly wider spacing produced higher size of plant height, leaf length and number of leaves. Bulb length, diameter and weight also the same trend in wider spacing. The weight of individual bulb of onion (23.52 g) was increased with the widest spacing (20x10 cm). On the contrary, yield ha–1 was the highest (16.65 t ha–1) at the closest spacing (7.5x7.5 cm) and the lowest (10.05 t ha–1) was at widest spacing (20x10 cm). But in closer spacing, bulb size was so small that was not suitable for the choice of consumer. On the other hand wider spacing produced the highest percentage (24.34%) multiplier bulbs that was not better for storing and consumers demand. So, in respect of economic point of view 15x10 cm spacing recommended in onion cultivation. It was found BARI Piaz-1 performed better in respect of yield and other parameters.