Abstract: Regeneration of multiple shoots via callus induction and organogenesis was achieved from cotyledon explants of chickpea (Cicer arietinum L.). Callus induction and shoot regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. Highest percentage (95) of callus formation was observed on MS+ 3.0 mg l–1 2, 4-D+3.0 mg l–1 BAP. The maximum percentage (40) of shoot bud formation was obtained on MS medium fortified with 2.0 mg l–1 BAP and 0.5 mg l–1 NAA with number of shoots per callus was 2.50. The regenerated shoots developed highest percentage (77) roots on ½ MS basal medium containing 1.0 mg l–1 IBA. Regenerated plants were successfully established in soil after acclimatization. Maximum survivability after 4 weeks of transplantation was achieved in 21 days old rooted shoots on garden soil.