Abstract: The seed oil of Abroma augusta Linn. was studied for biological and pharmacological properties. The oil was also screened for various in vitro biological and pharmacological activities including antifungal, antibacterial, insecticidal, phytotoxic and brine-shrimp cytotoxic activities. The oil exhibited remarkable phytotoxic activity against Lemna aeguinoctailis Welve. It was also explored to posses moderate antifungal activities against Trichophyton schoenleinii (56%) (human pathogens) and Microsporum canis (50%) (animal pathogen). The Abroma augusta seed oil did not shows the significant antibacterial, insecticidal activities and Brine shrimp cytotoxicity.