Abstract: The acrylamide derivative, 3-(4-hydroxy-phenyl)-N-[2-(4-hydroxy phenyl)-ethyl]-acrylamide (H-1) was screened against thirteen pathogenic bacteria for its antibacterial activities. The zones of inhibition produced by the test materials were found between 11 and 20 mm. The minimum inhibitory concentration (MIC) was also measured against Bacillus subtilis and Shigella dysenteriae and the value was found to be 128 μg/ml. The cytotoxic activity was also measured by brine shrimp lethality bioassay and the LC50 values of H-1 and standard ampicillin trihydrate were found to be 56.23 and 6.13 μg/ml.