Abstract: In order to compare the genetic variability of a hatchery and a wild population of rohu (Labeo rohita), four enzyme systems, PGM, GPI, MDH and LDH were studied using horizontal starch gel electrophoresis. The enzymes were found to be encoded by 6 gene loci: Ldh-1*, Mdh-1*, Mdh-2*, Gpi-1*, Gpi-2* and Pgm*. Within the six loci, Gpi-2*, Mdh-1* and Pgm* were found to be polymorphic. The mean number of alleles per locus and heterozygosity values (observed and expected) in wild population were 1.660 and 0.217 respectively which were higher than those observed in hatchery population (1.500 and 0.142 respectively). The relatively lower level of genetic variability of hatchery population could be attributed to a limited number of founders of the hatchery stock and inbreeding.