Abstract: The present study was conducted on maize, with the aim to work out a suitable surface disinfecting procedure for seeds and to know the best concentration of 2,4-dichlorophenoxyacetic acid (2,4 -D) for callus induction in N6 medium using var. EV-2097. Better disinfection of seeds was achieved with 30 minutes Clorox treatment (86 %). Maximum callus induction (47 %) was recorded with 2 mg l-1 followed by 3 mg l-1 (18 %) and 1 mg l-1 2,4 -D (16 %).