Abstract: This study aimed to determine the protective effects of intraperitoneally administered vitamin E, lipoic acid and linalool on the levels of fatty acid in guinea pig liver and muscle under oxidative stress induced by hydrogen peroxide. While the level of total unsaturated fatty acid in the vitamin E, lipoic acid and linalool groups significantly increased (p<0.01) as compared to the H2O2 group but its level slightly increased (p<0.05) as compared with the control, H2O2+ vitamin E, H2O2+ lipoic acid and H2O2+ linalool groups in liver. The level of polyunsaturated fatty acid (PUFA) in H2O2+ vitamin E, H2O2+ lipoic acid and H2O2+ linalool groups increased in comparison with H2O2, decreased in comparison with vitamin E and lipoic acid groups in both tissues. In muscle, the level of unsaturated fatty acid in the H2O2+ vitamin E, H2O2+ lipoic acid and H2O2+ linalool groups increased in comparison with H2O2 group, but its level in the same groups decreased in comparison with vitamin E, lipoic acid and linalool groups. Vitamin E, lipoic acid and linalool could be used in therapeutic approaches, aimed at limiting the damages from oxidation reactions in unsaturated fatty acids.