Abstract: The allelopathic effects of Eucalyptus leaf-litter water extract (ELWE) and crude leaf-litter powder (ECLP) were studied on growth activities, nutrient uptake and the rate of metabolite accumulation of broad bean (Vicia faba L.) and Maize (Zea mays L.). The results showed that the germination of two species (maize >broad been) was enhanced at a level of 1% ELWE and greatly reduced (broad bean > maize) at 5% level. The shoot and root lengths, total dry weight and relative growth rate (RGR) (shoot > root) were greatly reduced in broad bean than in maize. Similarly, the leaf area index (LAI) was significantly (p < 0.05) reduced in the two species. The allelopathic compounds released from ECLP significantly (p < 0.01) suppressed the uptake of N, P and K in the two examined species. Such reduction was not correlated to the concentration of nutrients in plant tissues but highly positively correlated (p < 0.01) with the reduction that occurred in dry matter production. The two species exhibit a tendency to accumulate amino acids; proline and sugars, which may be considered as an adaptive mechanism to increase the stress tolerance. The study indicates a firm conclusion for the possibility of Eucalyptus phenolic and terpenoid compounds phytotoxicity for the two examined species.