Abstract: The chromosomes were identified individually and standard karyotype of Lycopersicon esculentum cv. Oxheart was formulated. Roots were collected from germinating seeds and were pre-treated in a saturated aqueous solution of monobromonaphthalene. After iron-alum mordanting, standard hematoxylin method was followed. 2n = 24 chromosomes were found. Karyotype analysis was carried out following a quantitative method using scatter diagram technique. Only two chromosomes were identified individually and the rest were morphologically characterized. One of the two identified chromosomes had mean total length 3.1μ and mean arm ratio 1.3 whereas, the other one had mean total length 2.5μ and mean arm ratio 2.0. The length of the prometaphase chromosomes ranged from 1.3 to 3.1μ. The karyotype of the studied tomato genotype consists of 8 metacentric and 4 sub-metacentric chromosomes. One pair of satellited chromosomes was also observed.