Abstract: The duration of storage of frozen bull semen with acceptable quality without pouring any liquid nitrogen into 2-liter container was investigated under field conditions. One frozen straw of each of the 3 donor bulls was evaluated daily for 15 days with regard to sperm motility and normal sperm morphology with respect to acrosome, mid-piece and tail. When frozen semen straws were stored within 2-liter liquid nitrogen (LN) container without pouring any LN into the container up to 15 days of storage, the sperm motility and normal sperm morphology decreased gradually up to 10 days of storage and then the sperm motility and the normal sperm morphology decreased significantly (P<0.001). The motility did not differ significantly between bulls. However, the proportion of normal sperm morphology varied significantly between bulls (P<0.001). In conclusion, frozen bull semen straws can be stored in 2-liter liquid nitrogen (LN) container with acceptable quality for 10 days without any LN under field conditions in Bangladesh.