Abstract: Responsiveness of the variety Falgooni Tossa i.e. O-9897 (Chorcorus olitorius L.) in late jute seed production to Nitrogen (N), Phosphorus (P) and Potassium (K) was assessed in different Agro-Ecological Zones (AEZ) with increasing dose of N, P and K fertilizers. A highly significant effect was observed for plant height, number of branches/plant, number of pods/plant, pod length, number of seeds/pod, 1000 seeds weight and seed yield with the application of increasing doses of N fertilizer. There was no significant effect of increasing doses of P fertilizer on plant height, number of branches/plant, pod length and number of seeds/pod but a significant effect found for number of pods/plant, 1000 seeds weight and seed yield. No effect towards seed yield and yield contributing characters was observed with increasing doses of K fertilizer. Significantly high seed yields were found with the application of 100-20-20, 75-40-20 and 100-20-20 kg N-P-K/ha at Rangpur (AEZ No. 3b), Manikganj (AEZ No. 8d) and Kishorganj (AEZ No. 8b) respectively.