Abstract: The investigation was based on axillary shoot proliferation, culture multiplication and rooting of the in vitro generated and proliferated shoots of Annona. Different strengths of MS and MMS media showed the best result. For axillary shoot proliferation nodal explant from field grown mature plants showed the best results on MMS medium containing 0.2 mg/l BA(6-benzyl adenine) +0.1 mg/l NAA( α-naphthalene acetic acid) . The highest degree of axillary shoot proliferation from nodal explant was found 80% with 6.0±0.42 shoots per culture. Root forming performance of IBA(Indole-3-butyric acid) was found to be the best among three auxins (NAA, IAA/Indole-3-acetic acid, IBA) tested. The highest percentage of root formation was obtained with 2.00±0.21 roots per cutting on the medium containing 0.1 mg/l IBA. At the high concentration (0.1-0.2 mg/l) of all auxins callus formed from the cut margin of the micro cuttings. The regenerated shoots with well developed roots were gradually acclimatized and successfully transferred to the soil under natural conditions.