Abstract: Four different doses such as 50, 100, 200 and 400 mg estradiol-17β/kg feed were administered for 40 days on African catfish bearing an average length 5.1 mm and weight 4.7 mg. Growth, survival and sex-ratios were evaluated. The mean length of fry after hormone feeding phase were found to be more or less similar in all the treatments. The mean weight of fry of the group fed on diet containing 100 mg estradiol-17β/kg feed was found to be significantly higher than those receiving 50, 200, 400 mg estradiol-17β/kg feed and control. The groups of fry treated with 400 mg estradiol-17β/kg feed and control group showed lower survival compared to those with other doses of hormone. No negative effect on growth and survival of estradiol-17β was however, observed in this study. The doses of 100 mg and 50 mg estradiol-17β/kg feed resulted in 87.76% and 80.85% female fish respectively which were significantly (p<0.01) higher than that of the expected frequency of female fish in a normal population. The dose of 400 mg estradiol-17β/kg feed resulted in relatively lower frequency of female fish.