Abstract: The degradation of natural rubber in a continuous culture system using actinomycete has been investigated at three exposure times (30, 60 and 90 days) employing diagnostic matching as well as the evaluation of mechanical and chemical properties. The biodegradation was carried out in a packed bioreactor varying three operating parameters; temperature (20°C, 30°C, 40°C and 50°C) pH (5,6,7 and 8) and aeration rate (0, 0.3, 0.5 and 1 vvm). The actinomycete growth characteristics were also investigated and found to be the best in a minimal media with a concentration of 0.1 g/l glucose. The actinomycete was able to grow in both glucose and non-glucose environments. In diagnostic matching, the morphology of degradation was matched with actual conditions using scanning electron microscopy. The morphologies observed were; striations, asperities, voids and bulk degradation. The intensity of biodegradation runs parallel with morphology in the order thus; striations, asperities, voids and bulk degradation. Asperities were observed at 60 days and temperatures of 20-40°C, striations at 40°C and an exposure time of 60 days. The morphologies with change in rate of aeration were voids and bulk disintegration. Voids were observed for all samples at aeration rates of 0.3-1vvm with onset at 30 days exposure. The morphology of degradation for the change in pH was voids with onset at 30 days exposure time. The voids then grew with increase in pH as well as exposure times, but did not manifest as bulk degradation at the end of the exposure, at the higher temperatures. The morphology of degradation was matched to the mechanism, enabling a mapping of the process to be made.