Abstract: Enzyme with proteolytic activity was found in the germinating wheat seeds (Triticum aestivum L.,). The enzyme activity appeared after 40 h of dark germination and reached its maximum value after 72 h and then declined sharply during further germination. After ion exchange chromatography, three protein peaks ( pro-I, pro-II & pro-III ) showed proteolytic activity and the degree of purification attained nearly 29-42 fold. Properties like optimum pH and optimum temperature of pro-I, pro-II and pro-III were 7.1, 6.4, 6.8 and 45°C, 43°C and 42°C, respectively. Mg2+ and Mn2+ had no effect on enzyme activities while EDTA, urea, Ca2+ had an activating effect. On the other hand Hg2+ and Fe2+ had strong inhibitory action on the extracted enzyme. The enzymes were found to be homogeneous as judged by Polyacrylamide disc gel electrophoresis. Molecular weights of pro-I, pro-II and pro-III were 1.08, 1.04 and 99kD and Km values were found to be 0.027, 0.032 and 0.037 mM respectively.