Abstract: The experiment was carried out at Central Cattle Breeding Station and Dairy farm, Savar, Dhaka, and 3 sub- station and 9 points of Chandpur District in Bangladesh to evaluate the quality and fertilizing capacity of locally produced chilled and imported frozen semen. Motility, sperm concentration and mass activity of semen from different experimental bulls were almost similar. Quality of imported frozen semen was better than that of locally produced chilled semen in respect of motility, motile sperm/ Insemination dose and spermatozoa with normal head. Motility and pH value of semen decreased significantly for transportation and prolongation of preservation duration. Average conception rate of imported frozen semen (57.33) was found to be higher than locally produced chilled semen (45.33). But it was similar between imported frozen (57.33) and average of 1st & 2nd day preserved semen (57%).