Abstract: A comparison of the chemical and fatty acid composition between cvs. Hassawi rice (Oryza sativa L.) and Hassa No. 2 was made. Although other parameters of proximate analysis were not significantly modified, content of crude protein in cv. Hassa No. 2 decreased from 10.68% to 9.24%, whereas carbohydrates increased from 75.69% to 77.38%. In response to breeding, mineral composition was unchanged. Saturated fatty acids content significantly decreased with cv. Hassa No. 2, whereas, unsaturated fatty acids increased in comparison with cv. Hassawi. Palmitic, oleic, and linoleic acids were the most abundant fatty acids in both genotypes. Linolenic acid was significantly higher and palmitic acid was significantly lower in cv. Hassa No. 2 than in cv. Hassawi but myristic, stearic, oleic, and linoleic acid content were comparable. Apparently modifications were detected in the concentration of chemical constituents in seeds of Hassawi rice hybrid, the extent of which was dependent on the parameter being considered.