Pakistan Journal of Biological Sciences

Year: 2002 | Volume: 5 | Issue: 12 | Page No.: 1309-1310
DOI: 10.3923/pjbs.2002.1309.1310

Studies on the Biosynthesis of Enzyme Xylanase by Submerged Fermentation from Aspergillus niger GCBMX-45

Ikram-ul- Haq, Amna Ehsan, Waseem Ahmad Butt and Sikander Ali

Abstract: The optimization of cultural condition was studied for the production of xylanase by mutant strain of Aspergillus niger GCBMX-45. Submerged fermentation was carried out in 250 ml Erlenmeyer flasks. Medium containing (%w/v) NaNO₃, 0.1; Tween-80, 0.2; NH₄Cl, 0.1; KH₂PO₄, 0.1; MgSO₄.7H₂O, 0.03; CaCl₂.2H₂O, 0.1; wheat bran, 2 was used for xylanase production. The enzyme production was maximum (195 U ml^-1) at pH 4.5. One percent meat extract was added in medium as an additional organic nitrogen source.