Abstract: In the last tow decades Thermophilic Bacilli were classified as either B. coagulans or B. sterothermophilus, but it is becoming increasingly evident that they comprise a diverse group of organisms. In present study thermophilic Bacillus (gram positive, motile, spore forming) was isolated, characterized and identified from recreational thermal spring located along the Jordan Rift valley. The isolated strain was distinguished by the formation of terminal cylindrical spore, it was named Bacillus brevis. The optimum growth temperature for this strains was 570C. Studying thermostable proteases (from thermophilic Bacilli) as biocatalysts for industrial applications is going to be considered for future work.