Abstract: Patterns of morphological variation in Hordeum species were determined by metroglyph analysis which revealed apparently four clusters comprising more than one line out of 15 studied. Cluster I was comprised of Centinella and Conquest of H. vulgare. Cluster II was comprised of single line of H. nudum and four lines (API-19, BEL-34, BEL-72 and BTON-10) of H. vulgare. Cluster III and IV were consisted of BEL-4, BTYN-8 and BTYN-37 and BEL-16, BHV-1, BHV-91, BHV-95 and BHV-105 of H. vulgare respectively. These clusters showed distinct graduation for breadth of flag leaves i.e., low (cluster I), relatively low (cluster III), intermediate (cluster II) and high (cluster IV). The ray`s pattern for 11 morphological characters on the glyph among the clusters revealed a marked variation for the presence or absence of rays.