Abstract: A 50-days feeding trial was conducted with the fingerlings of rohu (Labeo rohita Ham.). using three diets A, B and C containing fish meal, mixed animal protein and mixed plant protein each having 2, 4 and 6 times feeding frequencies per day to observe the growth performance. The highest and lowest mean growth percent of 130.60 and 70.00 % was observed in fish fed on diet A and diet C with 6 times and 2 times feeding frequencies per day respectively. Food conversion ratio of 1.95 was significantly higher (p<0.05) in fish fed on diet C having 2 times feeding frequencies while the least value of 1.29 was obtained in fish fed on diet A having 6 times daily feeding frequency. Protein efficiency ratio ranged from 1.52 in fish fed on diet `C` with 2 times feeding frequency to 2.32 in fish fed on diet `A` with 6 times feeding frequency. Other growth parameters viz., specific growth rate apparent net protein utilization and apparent protein digestibility were also studied. The results of the present observation demonstrated that the growth performance of L. rohita were the best at 6 times feeding in a day by using either diet A or diet B.