Abstract: A total number of 355 samples including urine (179), pus (55), throat (43), stool (31), sputum (22), eye & ear (12) swabs, high vaginal swabs (10) and semen (3) were collected from patients to determine the presence or otherwise of Escherichia coli and to ascertain their antibiogram sensitivity pattern. The incidence of E. coli was highest in urine samples (22), followed by pathogenic E. coli (3) from stool samples and 2 isolates from wound and 1 from HVS, whereas the other samples did not yield any. In case of urine samples E. coli isolates were found to be highly susceptible to amikacin and norfloxacin while resistant to ampicillin. All E. coli isolates of stool samples were resistant to ampicillin, chloramphenicol and tetracycline whereas aztronam, cefotaxime and ofloxacin were 100% effective against E. coli. In case of pus samples, E. coli isolates were found to be susceptible to amikacin and tobramycin, but completely resistant to cefuroxime, ampicillin and cephradine. Incidence of E. coli was reported to be high in urine samples, with females more susceptible than males.