Abstract: Seeds collected from naturally grown Isabella grape cultivar (Vitis labrusca L.) were treated with 0, 500, 1000 and 1500 ppm GA3, sown into perlite + silt (2:1), peat moss and soil mixture (1:1:1-sand : manure : soil) in the beds maintained at 25°C, 30 °C and 35° C bottom heating with control (no bottom heating). The first germination occurred on 17th day after sowing of the seeds treated with 0 and 500 ppm GA3 and sown into the peat moss having a bottom heating temperature of 35° C. Germination ended within 35 days. Bottom heating caused to germinate the Isabella grape seeds earlier than the control application but did not affect the final germination rate. Finally, bottom heating had no positive effect on seed germination while perlite + silt (53.21%) and 1500 ppm GA3 treatments (51.72%) promoted the germination of Isabella grape seeds.