Abstract: Karyological characteristics of two specimens belonging to the superspecies Spalax leucodon (Nordmann, 1840) from two central Anatolian localities, Kayseri and Sivas-Gürün, were studied to provide insight into chromosomal variations. The two subterranean mole rats examined in this study had karyotypes with 2n=60, NF=78 and NFa=74. This karyotype has already been described at two sites in previous studies but given here for two new additional localities for the first time.