Abstract: Pseudomonas syringae pv. lachrymans were isolated from cucumber (Cucumis sativus) seedlings raised from seeds which were previously treated with Thiram or Captan. Five isolates were identified and used in pathogenicity test. Leaf inoculation gave the highest percentage of infection, followed by stem and the lowest percentage. The results highlight to some extent that the court of infection with P. syringae pv. lachrymans is the cucumber leaf. Further investigations are needed to study the interrelation between the location of infection and the translocation of the pathogen in the plant to confirm this results.