Abstract: A four-parent di-allel cross including CRIS-5A, CRIS-9, CRIS-52 and CRIS-54, cultivars of Gossypium hirsutum was analyzed for heterosis, heterobeltiosis and economic heterosis. The traits studied were the number of sympodia, bolls per plant, boll weight and seedcotton yield. Results revealed that seedcotton yield was dependent on number of bolls per plant (whenever the heterosis for number of bolls per plant went high heterosis for seedcotton yield was also increased). Four hybrids CRIS-52 x CRIS-54, CRIS-5A x CRIS-52, CRIS-52 x CRIS-9 and CRIS-54 x CRIS-52, exhibiting more than 70% of all types of heterosis were singled out.