Abstract: Exogenous sugars, especially sucrose is very essential for providing osmotic environment and nutrition to in vitro pollen grain germination. Pollen bursting is frequently observed in artificial medium lacking suitable sucrose concentration. Four sucrose concentrations 10, 20, 30 and 40% were tried for okra pollen germination. At 10% sucrose,majority of pollens brusted; however, at 20% sucrose, 80% ofpollen grains germinated by producing tube lengths in the range of 3000 to 4000 μm. At 30%, the pollen germination% and tube length declined by about more thantwice against 20% sucrose level. At 40% concentrations, pollen grains` germination and tube length reduced remarkably by giving only500 to 700 μm pollen tubes. Not only tube length retarded at these high concentrations but pollentubes also changed to more thickened and shortened structures.It was therefore observed that 20% sucrose is an appropriate concentration for in vitro okra pollen germination.