Abstract: Cotton is an economically important crop and experiments to determine its response to application of nitrogen fertilizer at proper time is of prime importance in research. Thus a field trial was conducted to evaluate the impact of nitrogen fertilizer application at various timings on seedcotton yield at CCRI, Sakrand. The treatments consisted of four timings of nitrogen application (planting, squaring, flowering and peak flowering). Cotton cultivar CIM-443 was planted during second week of May and 100-kilogram nitrogen fertilizer per hectare as urea was applied at various timings during the season. The results revealed that there is a significant impact of multiple split application of nitrogen fertilizer. Crop receiving 4-equal splits of nitrogen application at various growing stages of cotton crop proved better and gave better yields than the rest.