Abstract: Dry seeds of Citrullus colocynthis collected in 1982 and stored at 10°C, were extracted to investigation endogenous cytokinin content. An HPLC analysis was made and profile obtained revealed the presence of two isomers of zeatin (trans-zeatin and cis-zeatin), isopentyl adenine and isopentyl adenosine. Analysis showed very high content of trans-zeatin as compared to cis-zeatin. Isopentyl adenine and isopentyl adenosine were in negligible quantity.