Abstract: Studies were carried out to determine natural resistance of cotton varieties against insect pests. Population of thrips reached above threshold levels from 1st week to 3rd week of July in all the strains/varieties (CRIS-120, CRIS-126, CRIS-133, CRIS-134, CRIS-9 and NIAB-78) and needs to be sprayed to protect the crop from economic loss. CRIS-134 showed more tolerance against bollworm damage followed by CRIS-133. Maximum seed cotton yield was obtained from CRIS-120, followed by NIAB-78 and CRIS-126 respectively. Regular pest scouting should be carried out in the field and when pest populations reach at threshold levels, the crop should be sprayed with appropriate pesticides accordingly.