Abstract: Abaxial and adaxial leaf epidermal anatomy of four related species having morphological resemblances have been studied. These taxa showed variation in short and long cells, silica bodies, macro and micro hair, presence or absence of stomata and shape of subsidiary cells. Most of these characters are diagnostic and have been used for making keys. Hence, on the basis of leaf epidermal anatomy we can distinguish among Cymbopogon citrates (DC.) Stapf, Cynodon dactylon (L.) Pars, Panicum summatrense Roeth ex Roem & Schult and Viteveria zizanoides (L.) Nash.