Abstract: Botryodiplodia theobromae Pat., is the causal organism of crown rot disease of banana. This fungus grew and sporulated at 10-40 °C, the optimum being 25-30 °C and the highest mycelial growth (78-90 mm) and sporulation (27-38 conidia/0.01 ml) were observed on PDA. There was no growth of the organism at 10 and 45 °C. Light was not necessary for growth, but it enhanced the sporulation. Maximum pigment formation occurred on PDA (Black 75% and White 25%) and minimum on PCM (Black 10% and White 90%). Formation of pycnidia initiated on 6th day in all the media, except Sabouraud`s medium. Maximum pycnidia formation occurred on Czapek`s (35/plate) medium and minimum on PCM (6/plate) medium. In case of glucose added PA medium, maximum pigment formation occurred on 35 gm of glucose added PA medium and minimum on PA medium. The highest mycelial growth was observed on PA medium (168 mm) and the lowest (54 mm) on 35 gm of glucose added PA medium. The highest number of pycnidia formed on PA medium (38/plate) and the lowest on 30gm glucose added PA medium (3/plate). There was no pycnidia formed on 35 gm glucose added PA medium.