Abstract: Anatomical investigation has been made on the stem of lignosus bean (Dipogon lignosus (L.) Verde.) at different stages of growth. The epidermis is single layered consisting of small and large cells. The epidermis bears multicellular hair and glandular trichomes. Beneath the epidermis there are 5-6 layers of cortical cells in the stem. The primary vascular tissue appears after the elongation of the first internode of the stem of lignosus bean. The internode between cotyledonary node and first leaf is considered as the first internode of the stem. The vascular bundles are collateral and arranged in a ring. The secretory cells devoid of tanniniferous contents have been observed in the phloem region of the younger stem but not in older ones. The cambium initiates in the primary vascular bundle between xylem and phloem at the basal part of the stem of 7 days old plant. The cambium is at first confined to the fascicular region. Subsequently it extends into the interfascicular region forming a complete cambial ring. After the formation of fascicular cambium it gives rise to secondary xylem adaxially and secondary phloem abaxially. Some vessel members are paired while the others are solitary. Most of the paired vessels are radially arranged. The paired vessel members are more in number in the mature stem compared to that of the younger stem. The parenchyma covers the major area of the secondary xylem. The sclerenchymatous band abaxial to all large and small vascular bundles is discontinuous. The pith resembles a typical dicotyledonous stem. The phellogen appears in the deeper cortex and gives rise to 4-5 layers of cork cells abaxially and 3-4 layers of phelloderm adaxially.