Abstract: The ability of explants of gladiolus cv White Prosperity to form plantlets and cormlets was investigated in vitro studies. Maximum production of shoots per explant was obtained on basal medium of Murashige and Skoog (1962) containing 18 μM 6-Benzylaminopurin (BAP) and 36 μM Indole-3-butyric Acid (IBA). Callus formation was observed when in vitro cultures were maintained for 16-18 weeks. When different explants were used for their response to regenerate, maximum number of shoots per culture was achieved from nodal buds. In order to allow initiation of root formation, the shoots were transferred to one half strength Murashige and Skoog medium containing 2.5 μM, Naphthalenacetic Acid (NAA) or 19 μM Indole -3-butyric Acid (IBA). Roots developed readily within 4-5 weeks on either of the two media. However, all plantlets grew with better survival in medium containing NAA as harmonal supplement than in media containing IBA. Further MS medium containing 6 percent sucrose facilitated maximum growth of cormlets to allow their use as propagation material in vivo.