Abstract: Flow cytometry was successfully used for screening ploidy levels in a large population of in vitro induced autopolyploids of the Musa acuminata ssp. malaccensis a wild banana. Use of zygotic embryo culture for large production of banana seed progenies and improved media compositions has facilitated the process of polyploidy induction. Treating shoot apices with 0.5% colchicine in combination with 2% DMSO for 2 h successfully produced tetraploids in Musa. Tetraploids produced were further confirmed through chromosomal counts.