Abstract: A field experiment was conducted to study the effect of timing of the last dose of split nitrogen application on agronomic traits and nutrient contents of sugarcane. The study was carried out at the National Agricultural Research Centre Islamabad during 1996-97. Application timing of the last dose of nitrogen did not affect early stalk population, millable canes, stalk height, stalk girth and total soluble solids (TSS). Application of last dose of N at early stage (last week of March) produced significantly lower cane yield than other timings of N application. Maximum cane yield of 146 t ha–1 was produced when the last dose of N was applied during the first week of June. Cane yield produced with the application of last dose of N either the mid May or the first week of July produced statistically similar cane yield that was produced with the application of last dose of N during first week of June. Sugar recovery was significantly decreased with the late application (July 1st week) of the last dose of N. Maximum sugar yield (15.70 t ha –1 ) was produced when the last dose of N was applied during the middle of May. P, K, Zn and B contents were not affected with the timing of application of nitrogen. However, Mg contents of the tissue increased significantly as the application of the last dose of nitrogen was delayed.