Abstract: This study was undertaken to investigate the persistence of Avian Adenovirus-4 (AAV-4) in broiler breeders after the infection of Angara Disease (AD) and also to study its transmission pattern in the progeny. In the affected breeders the presence of AAV-4 was detected in organs of embryonated eggs and egg albumen derived from breeders at 30, 37 and 44 weeks of age, using Dot- ELISA. Shedding of the virus was found to be at its peak at the start of the laying period, decreasing successively thereafter. It has been proposed to vaccinate the breeders against AD to protect the progeny and eliminating the carriers so as to prevent the virus from circulating in the population.