Abstract: The degradative activity on various raw starches by enzyme from Synnematous sp., an endophytic fungus was studied. The enzyme hydrolyzed raw starches to produce maltose and glucose. Maximum maltose and glucose were produced from raw rice and tapioca starch were 18.8 and 3.6%, respectively. The yields were dependent on accessibility of granules surface to be attacked by the enzyme. The results of this study suggest that enzyme from Synnematous sp. has potential for the production of glucose and maltose using raw starches as substrates.