Abstract: A ten months investigation was conducted to know the status of bacterial flora in the farmed hybrid catfish (Clarias hybrid) and respective pond water from the fish pond of a commercial fish farm of Bangladesh. The average total load in pond water, slime and kidney of fish varied from 2.1×103 to 4.1×106 CFU/ml of water, 5.6×103 to 4.4×109 CFU/g of slime and 1.8×101 to 2.7×104 CFU/g of kidney. Coryneforms and Flavobacterium were the dominant bacteria in the observed fish-organs and pond-water. Higher bacterial load was recorded in the months of September and October. Sensitivity of the isolated Aeromonads and Pseudomonads to various antibacterial agents was determined. Higher percentage of resistant Aeromonads and Pseudomonads were observed against oxytetracycline followed by erythromycin. On the contrary, the percentage of resistant Aeromonads and Pseudomonads was lower against oxolinic acid.