Abstract: Antagonism between flax rhizobacteria and R. solan, the causal of flax seedling blight, was studied in vitro. Pseudomonas flurorescens, Pseudomonas cepacia and Pseudomonas sp. were the most effective antagonists. Linear growth of R. solani was inversely proportional to the concentration of the culture filtrates of Pseudomonas strains. Individual strains as well as their mixtures significantly increased the percentage of surviving seedlings in greenhouse and field trials; however, the mixtures were much more effective than the individual strains. Moreover, the mixtures were more effective in increasing seed yield and straw yield in field trials.