Abstract: Acid phosphatase is a hydrolytic enzyme which catalysis the hydrolysis of variety of phosphate esters that may involve in many biochemical steps. During present investigations, qualitative analysis of acid phosphatases from soluble and wall bound fraction was performed from different tissues of four varieties of mung bean. It was detected that number of bands varies from 1-6. Acid phosphatase (AP-II and AP-III) were present in leaves, flowers and pods whereas AP-V was specific to leaves and pods. More number of soluble acid phosphatase was observed in younger stages of leaves and pods, it decreased at the older stages. In case of wall bound fraction, young leaves and flowers showed lesser number of bands whereas an increase in the total number of bands was prominently seen as the growth proceeds.