Abstract: Newly developed strain GH-3 was tried against commercial variety Shaheen in sowing date trial over a period of three years to assess the optimum sowing time of the strain under Ghotki conditions. Accordingly 15th May was recorded as optimum sowing time for this area as maximum yield of 1979 kg ha–1 was obtained followed by 1st May and 1st June sowings that produced 1516 and 1491 kg ha–1 seedcotton yield respectively. The lowest yield (1160 kg ha–1) was obtained when the crop was sown on 15th June followed by 1278 kg ha–1 when sowing was done on 15th April. Strain GH-3 out-yielded commercial variety Shaheen irrespective of seasons and sowing dates.