Abstract: A field experiment was designed to assess the yield performance of four genotypes under five plant spacings (15, 22, 30, 87 and 45 cm) during 1997 and 1998 years at CCRI, Sakrand Sindh. On an average, highest seed cotton yield of 2121 kg ha–1 was obtained when the plant-to-plant distance was maintained at 22 centimeters followed by plant spacing of 15 centimeters where seed cotton yield of 2055 kg ha–1 was harvested. The lowest yield of 1720 kg ha–1 was produced from plant to plant distance of 45 centimeters. The highest yielding variety was CRIS-134 that produced 2067 kg ha–1 followed by CRIS-9 with 1944 kg ha–1 seed cotton yield.