Abstract: An experiment was carried out at Brackishwater Station, Bangladesh Fisheries Research Institute, Paikgacha, Khulna from July to August, 1999 to assess the effect of feed on larval survival of Macrobrachium rosenbergii. Three treatments viz. Artemia nauplii plus egg custard (T1), Artemia nauplii only (T2) and rotifer-000 only (T3) were taken for the study each with three replicates. The experiment was conducted in celluloid tanks with M. rosenbergii larvae at a density of 50 nos./litre of water. Highest average survival rate (30.0%) was found in T1 and the lowest (4.5%) obtained in T3. Analysis of variance showed that the difference in larval survival under different treatments were significantly different (p<0.01). The results obtained implied that there is a immense potentiality for increasing freshwater prawn seed production through closed water system by using Artemia nauplii plus egg custard as feed.