Abstract: The study was conducted at Bangladesh Agricultural University Dairy Farm, Mymensingh to find out the skin thickness in different regions of body of dairy cows and the relationship of skin thickness to milk yield. Twenty five crossbred milking cows of indigenous and Sahiwal were selected for experiment. The skin thickness was measured by Vernier calipers. The skin thickness of the same animals is varies at different regions of the body. The average skin thickness of neck, dewlap, chest, abdomen and hindquarter were 3.33, 4.12, 2.93, 4.23 and 4.20 mm, respectively and the average skin thickness was 4.20±0.90 mm at the five different regions and the milk yield were negative and was significant (p<0.05). The results of the present study indicated that 29% of the variation in milk yield of cows depend on skin thickness and 71% depends on another factors. Form the study it was found that milk production of low skin thickness group was higher then that of medium and high skin thickness group. This may be a fact that low skin thickness cows did not deposited extra fat in their body and utilized most of her energy for milk production.