Abstract: Hepatitis C virus causes acute and chronic hepatitis. It has a high propensity for transition to chronicity, In some cases over 50% patients developed chronic hepatitis. We have undertaken studies on protein/enzyme of serum of both normal and patient with chronic liver disease. Serum was analysed enzymatically and electrophoretically using reducing and non-reducing condition. It is observed that a protein of 14 Kda may be missing or in t race amount in serum of patients with chronic liver disease. Therefore it may be possible that this may be a cause of chronic liver disease. Further research is in progress for identification and characterization of missing protein.