Abstract: An experiment was conducted at the Bangladesh Agricultural University, Mymensingh, in the rabi season (November to April) to study the effect of variety and planting arrangement on nutrition and nutrient uptake in maize. Four maize varieties-Barnali, Khoibhutta, Mohor and Shuvra were planted in two plating arrangements-one plant per hill 25 cm apart (P1) and two plant per hill 50 cm apart (P2) in a constant row spacing of 75 cm. A split-plot design was used with three replications by placing variety in the main plot and planting arrangement in the sub plot. Results showed that both variety and planting arrangement had significant influence on nutrition and nutrient uptake in maize. Shuvra gave the highest grain yield (6.20 t ha–1) followed by Manor 15.84 t ha–1) and Barnali (5.59 t ha–1). Stalk yield was the highest (7.21 t ha–1) from Mohor. One plant per hill (P1) produced more grain and stalk yield (5.72 t ha–1 and 6.45 t ha–1, respectively) than P2 (5.41 t ha–1 and 5.98 t ha–1 respectively). Nutrient content in grain differed significantly among the varieties but similar in both the planting arrangements (P1 and P2). In general, higher nutrient uptake by a variety appeared to correspond to its high grain yield. Planting arrangement also had influence on nutrient uptake. One plant per hill 25 cm apart caused more uptake than 2 plants per hill 50 cm apart. This study showed that the varieties Shurva and Mohor and one plant per hill 25 cm apart had higher nutrient uptake efficiency that resultant higher grain and stalk yield.