Abstract: Studies were conducted to observe the effect of L-tryptophan on growth and yield of tomato. It has been concluded that L-tryptophan improved the vegetative and reproductive growth of tomato. Higher concentrations of Ltryptophan i.e., 10–4 ML–1 and 10–1 ML–1 were found much suitable to boost up stem height, number of leaves per plant. Lower concentrations of L-tryptophan i.e., 10–2 ML–1 and 10–3 were found favourable to improve reproductive characters i.e., number of trusses, fruits per truss and per plant, weight of fruit and number of seeds. TSS was lowered with the application of the chemical and ascorbic acid was enhanced with 104 ML‾1 concentration of L-tryptophan.